August 17-31, 2016
Thursday18Aug
Protein folding and dynamics from single-molecule measurements
| Date | Thursday, August 18, 2016 |
| Time | 14:00 |
| Location | Gerhard M.J. Schmidt Lecture Hall |
| Lecturer | Prof. Dmitrii E Makarov Department of Chemistry, University of Texas |
| Organizer | The Clore Center for Biological Physics |
| Contact | |
| Details | Special Joint Seminar: The Department of Structural Biology & Clore Seminar on Soft and Biological Physics |
Thursday18Aug
"Protein folding and dynamics from single-molecule measurements"
| Date | Thursday, August 18, 2016 |
| Time | 14:00 - 15:00 |
| Title | Special Joint Seminar |
| Location | Gerhard M.J. Schmidt Lecture Hall |
| Lecturer | Prof. Dmitrii E Makarov Department of Chemistry University of Texas |
| Organizer | The Clore Center for Biological Physics |
| Contact | |
| Abstract | In the past two decades, single-molecule experiments have evolved from being state-of-the-art prof-of-principle demonstrations to nearly routine tools of modern biophysics, enabling one, for example, to monitor molecular processes directly as they unfold in the cell. Yet because of the relative sluggishness of the common probes, deciphering single-molecule signals to infer molecular dynamics remains an elusive goal. In this talk I will report on recent joint efforts of my group with experimentalists toward this goal using the example of one of the most fundamental problems in biophysics, protein folding. I will discuss how intrinsic protein motion can be deduced from random photon sequences in single-molecule fluorescence resonance energy transfer experiments or from the movement of micrometer-sized force probes in single-molecule pulling studies. I will further describe some of the new lessons about protein folding and dynamics learned from such studies.
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Wednesday24Aug
G-INCPM - Special Seminar - Prof. Matthias Nees, Institute if Biomedicine, Univ. of Turku, Finland - "Combining Speed of Analysis with Complex Tissue Models for Physiologically Relevant High-Content Screening"
| Date | Wednesday, August 24, 2016 |
| Time | 11:00 - 12:30 |
| Location | Edna and K.B. Weissman Building of Physical Sciences Auditorium |
| Lecturer | Prof. Matthias Nees Institute of Biomedicine Univ. of Turku Finland |
| Organizer | Department of Biomolecular Sciences |
| Contact | |
| Abstract | In vitro model systems used in drug discovery typically do not address the complex architecture of human disease tissues. Only few approaches aim to faithfully recapitulate the complexity, heterogeneity and cellular dynamics e.g. in epithelial tissues and carcinomas. The most important aspects relate to the (tumor-) microenvironment, including cell-cell and cell-matrix interactions, inflammation and the role of stromal components. All of these elements can have a significant, but often underestimated impact on differentiation, normal and abnormal tissue functions, or drug response versus drug resistance. The basis for performing informative high content screening campaigns with such complex tissue models in vitro is access to fast, automated image analysis. We have developed a software platform (AMIDA, Automated Morphometric Image Data Analysis) that captures a large number of morphometric features in an unsupervised fashion. This approach enables us to capture much of the inherent complexity and dynamics of microtissues, yet still allows high experimental throughput. This screening platform is ideally suited for investigating a broad spectrum of defined, biological questions in drug discovery as well as personalised medicine. Technology and screening platform are applicable for multiple types of research, such as quantitatively measuring the response of primary cancer cells or cell lines to drugs, siRNAs or other perturbations. Image analysis algorithms can also be adapted towards specific applications in neurodegenerative diseases, stem cell research, and to quantitate the interaction of epithelial cells with immune, adipocytes or mesenchymal stem cells. |
Thursday25Aug
Full humanisation of the mouse immunoglobulin loci
| Date | Thursday, August 25, 2016 |
| Time | 10:00 |
| Location | Wolfson Building for Biological Research Auditorium |
| Lecturer | Prof. Allan Bradley Kymab, Cambridge MA |
| Organizer | Department of Neurobiology |
| Contact | |
| Details | Host: Prof. Michal Schwartz michal.schwartz@weizmann.ac.il tel: 2467 |
| Abstract | Professor Bradley is internationally recognized as a pioneer in developing the techniques, technology and tools for genetic manipulation in the mouse over more than 3 decades. He served as Director of the Welcome Trust Sanger Institute from 2000 to 2010. He was honored by election to the fellowship of the Royal Society in 2002. Among many projects that Dr. Bradley has established and led, is the international project to systematically knockout all genes in the mouse genome, the most ambitious use of ES-cell technology ever attempted. Over the last 30 years, Dr. Bradley has authored more than 280 publications. In his lecture, Dr. Bradley will be describing the scientific history and the technology behind the creation of the Kymouse strains which are transgenic for the total human immunoglobulin gene diversity. The platform provides a valuable means to isolate therapeutic monoclonal antibodies. Kymab has also developed single B cell-based methods to capture both the heavy and light chains of antibodies at scale. Combined with deep sequencing of millions of B cells we are able to build networks of histories of B cell families which we use to isolate rare antibodies with unique properties. The combined use of Kymouse with B cell network analysis, facilitates vaccine antigen discovery and predictive pre-clinical assessment of candidate vaccine antigens prior to clinical trials in humans.
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